Table 1. Oligonucleotide primer sequences used to detect the gyrA and parC genes in the E. coli isolates.
Gene | Primer name | Primer sequence (5΄- 3΄) | Annealing temperature (°C) | Amplicon size (bp) | References |
---|---|---|---|---|---|
gyrA | gyrA-F | TGGATTATGCGATGTCGGTCAT | 56°C | 696 | This study |
gyrA-R | TTTTGGCGTCAACTTCCACTTC | ||||
parC | parC-F | TGCCGTTTATTGGTGATGGTCT | 56°C | 477 | This study |
parC-R | GAGCCACTTCACGCAGGTTAT |