Long PCR amplification with F4-R2 primers of isolates Cer002t (haplotype Ia, lanes 1); GF266 (haplotype IIa, lanes 2); 1.T1 (haplotype Ib, lanes 3); and Tail (haplotype Ib, lanes 4) digested with HindIII, MspI, and RsaI. Lane M, 1-kb ladder (Gibco BRL Ltd.).