TABLE 2.
Oligonucleotide primers used in this investigation
| Primer | Sequence | Primer length (bp) and positiona |
|---|---|---|
| F1 | 5′-GCAATGGGTAAATCGGCTCAA-3′ | 21 23737–23757 |
| R1 | 5′-AAACCATAAGGACCACACAT-3′ | 20 24835–24854 |
| F2 | 5′-TTCCCTTTGTCCTCTACCGAT-3′ | 21 13619–13639 |
| R2 | 5′-TTACGGCGGTTTAGCACATACA-3′ | 22 14688–14667 |
| F3 | 5′-ATGGTAGAGCGTGGGAATCAT-3′ | 21 2892–2912 |
| R3 | 5′-AATACCGCCTTTGGGTCCATT-3′ | 21 4199–4179 |
| F4 | 5′-TGGTCATCCAGAGGTTTATGTT-3′ | 22 9329–9350 |
| R4 | 5′-CCGATACCGATACCAGCACCAA-3′ | 22 10292–10271 |
a
Primers were synthesized by Cruachem Ltd. The indicated positions refer to primer position on the P. infestans mitochondrial genome in relation to the origin (5′ end of the large subunit of the mitochondrial rRNA gene).