TABLE 3.

Amplification of P1–P4 PCR products from nine Phytophthora species

Species	Isolate	Origin (source, country)	Collectora	Primerb
				P1	P2	P3	P4
P. capsici	Pc34	Tomato, Taiwan	A	++	+	++	++
P. citrophthora	BP265	Cocoa, Brazil	B	++	+	++c	+
P. colocasiae	PT265	Taro, China	C	+	+	++	++
P. cryptogea	77/6	Tomato, United Kingdom	D	++	−	++	−
P. drechsleri	P208	Capsicum, Mexico	E	++	+	++	+
P. fragariae var. rubi	R6	Raspberry, United Kingdom	F	++	−	++	−
P. megasperma	17	Juniper, United States	G	++	−	++	−
P. nicotianae	BT113	Tomato, United Kingdom	D	++	+	++	++
P. palmivora	7298	Soil, PNG	H	++	−	++	+

^aCollectors are indicated as follows: A, Y.-H. Huang (AVRDC, Tainan, Taiwan); B, T. Dakwa (New Tafo, Ghana); C, K. M. Zhang (Hainan, China); D, P. Smith (GCRI, Littlehampton, United Kingdom); E. J. Galindo (Chapingo, Mexico); F, J. Duncan (SCRI, Dundee, United Kingdom); G, E. Hansen (Oregon State University, Oregon); H, F. Arentz (Bulolo, Papua New Guinea). 

^bThe amount of product generated at an annealing temperature of 53.5°C with each of the primer pairs is indicated as follows: ++, amount of amplification product similar to that obtained with P. infestans; +, clearly detectable PCR product but bands significantly weaker than for P. infestans; −, no detectable PCR product. 

^cAn additional band was also amplified.