Fig 7: The Osteocyte transcriptomes from male and female mice are distinct when challenged with a low calcium diet.

A: Volcano plot showing the significantly regulated genes between WT female low-calcium diet-fed (WT F) and WT male low-calcium diet-fed mice (WT M) osteocyte transcriptome.

B: Volcano plot showing the significantly regulated genes between KO female low-calcium diet-fed (KO F) and KO male low-calcium diet-fed mice (KO M) osteocyte transcriptome.

C: Heat map showing the differentially expressed genes among WT male low-calcium diet-fed mice (WT M), KO male low-calcium diet-fed mice (KO M), WT female low-calcium diet-fed (WT F), and KO female low-calcium diet-fed (KO F) osteocyte transcriptome.

D: Gene set enrichment analysis of Gene Ontology (GO) analysis of the significantly regulated genes between WT female low-calcium diet-fed (WT F) and WT male low-calcium diet-fed mice (WT M) osteocyte transcriptome, and between KO female low-calcium diet-fed (KO F) and KO male low-calcium diet-fed mice (KO M) osteocyte transcriptome. The figure shows the union of the top 10 GO terms of each analysis. If a term in the union, besides the top 10, is also significant (adjusted p-value less than 0.05 was used for GO analysis) in an analysis, it is also included in the figure.

The latter group in the figure’s title is the reference group. n=2–3/group. For DEG analys unadjusted p-value <0.01 was used.