Figure 4: SC-D2 tolerates domain replacement with mFPs and can be used to make polymeric fluorescent antibodies for antigen specific imaging.

(A) The SEC chromatogram of cSC^mCherry, showing a monodisperse peak, followed by the absorption spectra of 1μM cSC^mCherry, and fluorescence spectra of cSC^mCherry upon excitation with 560nm. (B) Schematic showing the assembly of surface layer proteins after the Slp A is cleaved to form high molecular weight (HMW) and Low molecular weight (LMW) fragments, the latter of which forms the outermost surface of the bacteria and is solvent accessible (PDB: 7QGQ). (C) Analytical SEC showing the binding of mFcα-CD5SLP binding to LMW-SLP. (D) Brightfield and flourescent microscopy images of SLP-coated latex beads incubated with the indicated molecules diagramed at the top. Scale bars are given in the top left comer of each image and are 150μm. See also Figure S4.