Figure 4. G6PD-deficiency increases reactive oxygen species.

A. Immunostaining for lipid peroxidation marker 4-hydroxynonenal (4-HNE) in 8-week-old Ptf1a^Cre/+, KC;G6pd^wt and KC;G6pd^mut pancreas. Scale bar = 1000μm.

B. Quantification of 4-HNE staining. Each datapoint represents one mouse. n = 3 mice per genotype.

C. Bright field images of ex vivo acinar cell cultures from KC;G6pd^wt and KC;G6pd^mut mice at 2 and 3 days. Cells were treated with mock (vehicle) or 1mM glutathione ethyl ester (GSHee), a cell permeable glutathione. Scale bar = 250μm.

D. Quantification of acinar cells undergoing ADM in ex vivo cultures at 2 days and 3 days. Grey bars are KC;G6pd^wt cells mock treated, blue bars are mock-treated KC;G6pd^mut cells, green bars are KC;G6pd^wt and KC;G6pd^mut cells treated with 1mM GSHee. Each datapoint represents one mouse. n = 3 mice per genotype.