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[Preprint]. 2024 Sep 4:2023.11.07.566024. Originally published 2023 Nov 7. [Version 3] doi: 10.1101/2023.11.07.566024

PMC10659360.1; 2023 Nov 7
PMC10659360.2; 2024 Jun 1
PMC10659360.3; 2024 Sep 4

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Figure 3. — Midface development requires dact1 and dact2. A) Representative brightfield images of wildtype and dact1/2−/− compound mutants at 4 dpf. 100 individuals were analyzed from a dact1/2+/− double het cross. Lateral and ventral views show dact1/2−/− compound mutants have a hypoplastic midface, medially displaced eyes, and a displaced lower jaw. B) Representative flat-mount images of Alcian blue stainedethmoid plate (EP) and visceral cartilage (VC) elements from 4 dpf wildtype and dact1/2−/− compound mutants. dact1/2−/− mutants have a rod-shaped EP with no distinct lateral and medial elements. No obvious differences were found in dact1/2 mutant VC. C) Representative brightfield image of 4 dpf wildtype and dact1/2−/− mutant. Bar indicates vertebral spine length. Scale bar: 100 μm. D) Representative images of Alcian blue stained dact1/2−/−, wnt11f2−/−, and wnt11f2−/−,dact1/2−/− compound mutants. Embryos resulted from a dact1+/−,dact2+/−,wnt11f2+/− in-cross. Lateral and ventral views show similar craniofacial phenotypes in each mutant. E) Representative flat-mount images of Alcian blue stained EP show a similar phenotype between dact1/2−/−, wnt11f2−/−, and wnt11f2−/−,dact1/2−/− compound mutants. Scale bar: 200 μm.