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[Preprint]. 2024 Jan 3:2023.09.28.559989. Originally published 2023 Sep 28. [Version 3] doi: 10.1101/2023.09.28.559989

Figure 4. Cleavage of chicken GSDMA leads to cell lysis.

Figure 4.

(A.) Measurement of GFP fluorescence in HEK293T/17 cells co-transfected with GFP and either full length (FL) chicken GSDMA or its N-terminal (NT) pore-forming domain after 48 hours. Each point represents a separate transfection experiment. ****, p<0.0001 by student’s t-test. (B.) Cell lysis measured by LDH release in HEK293T/17 cells co-transfected with chicken caspase-1 and either WT or D244A chicken GSDMA. Gasdermin constructs in this study do not encode the 3xFLAG domain. Each point represents a separate transfection experiment. **, p<0.01; ****, p<0.0001 by 2 way ANOVA with Tukey’s multiple comparisons correction. (C.) Images of HeLa cells after transfection with the N-terminal domain of chicken GSDMA. Transfected cells display classic pyroptotic ballooning. SYTOX green staining of the nucleus indicates permeabilization of cell membranes.