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[Preprint]. 2024 Jan 3:2023.09.28.559989. Originally published 2023 Sep 28. [Version 3] doi: 10.1101/2023.09.28.559989

Figure 6. GSDMA from non-mammals are cleaved by caspase-1 and SpeB.

Figure 6.

(A-C.) FLAG blot of HEK293T/17 lysates after co-transfection of A. mississippiensis, A. carolinensis, or M. unicolor GSDMA and caspase-1 from chicken, human and mouse. M. unicolor is an amphibian despite sharing morphological similarity to a snake. (D.) FLAG blot of HEK293T/17 lysates after co-transfection of platypus GSDMD or GSDMA with caspase-1 from chicken, human and mouse. (E.) FLAG blot of HEK293T/17 lysates after incubation with SpeB. Total protein captured by Ponceau staining for this blot.

figure supplement 1. Amphibian expression of CASP1, GSDMA and GSDME.