Figure 1. Loss of Dlg1 in mouse kidney cells leads to elongated cilia.
(A) H&E staining of representative kidney sections from wildtype and Pax3Cre-Dlg1F/F mice. (B, C) Immunofluorescence staining for cilia (acetylated tubulin, yellow) and quantification of ciliary length in kidney sections of wildtype and Pax3Cre-Dlg1F/F mice. * denotes P<0.05. (D) Western blot analysis of total cell lysates of the indicated mCCD cells lines using antibodies against DLG1 and GAPDH (loading control). Molecular mass markers are shown in kDa to the left. (E) Representative image of transwell filter-grown mCCD cell lines (mCh-DLG1: mCherry-DLG1). Cilia were visualized using acetylated tubulin antibody (AcTub, magenta), cell-cell contacts were visualized with E-cadherin antibody (green) and nuclei were stained with DAPI (blue). (F, G) Quantification of ciliary length (F) and frequency (G) in the indicated transwell filter-grown mCCD lines. Ciliary length and ciliation rate was measured using the fully automated MATLAB script. Graphs represent accumulated data from three individual experiments, and statistical analysis was performed using Mann-Whitney U test (unpaired, two-tailed). Error bars represent means ± SD. **, P<0.01; ****, P<0.0001; ns, not statistically significant.