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[Preprint]. 2024 Mar 14:2023.11.10.566524. Originally published 2023 Nov 10. [Version 3] doi: 10.1101/2023.11.10.566524

PMC10659422.1; 2023 Nov 10
PMC10659422.2; 2023 Dec 15
PMC10659422.3; 2024 Mar 14

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Figure 7. — (A, B) Immunoprecipitation with anti-GFP beads was performed in HEK293T cells transiently expressing FLAG-Myc-SDCCAG3 (A) or FLAG-IFT20 (HTF-IFT20) (B) together with the indicated GFP-fusions. Input and pellet fractions were subjected to SDS-PAGE and western blot analysis using antibodies against FLAG or GFP, as indicated, and GFP expressed alone was used as negative control. Molecular mass markers are indicated in kDa to the left. (C) Structural prediction for the complex between MmSDCCAG3 (yellow) and MmIFT20 (cyan) in cartoon representation (upper panel). The structure is predicted to be an anti-parallel hetero-dimer coiled coil. The lower panel includes IFT54 showing its binding to IFT20 is mutually exclusive with binding of SDCCAG3 to IFT20. (D) Proposed model for how DLG1 promotes ciliary trafficking of SDCCAG3, IFT20 and PC2. Based on [7, 9] and data presented in the current study. CRE, common recycling endosome.