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[Preprint]. 2024 Sep 18:2023.11.10.566612. Originally published 2023 Nov 10. [Version 2] doi: 10.1101/2023.11.10.566612

Figure 1: Tick EVs affect hematophagy and survival.

Figure 1:

(A) Graphical illustration of experimental design. (B-M) Vamp33 siRNA (siV33) (red) or vamp33 scramble control (scV33) (blue) microinjected nymphs were placed on C57BL/6 mice and allowed to feed for 3 days. On day 3, ticks were harvested and assessed for fitness measurements. Efficiency of Vamp33 silencing and tick attachment, weight, and survival curves for (B-E) I. scapularis, (F-I) A. americanum and (J-M) D. variabilis. Graphs represent at least three independent experiments combined. Statistical significance shown as *p<0.05, **p<0.01, ns = not significant was assessed by t test (B, F, J); Fisher’s exact test (C, G, K); Mann Whitney test (D, H, L); and Log-rank (Mantel-Cox) test (E, I, M).