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[Preprint]. 2023 Oct 6:2023.10.06.561273. [Version 1] doi: 10.1101/2023.10.06.561273

Figure 4. SpoVK interacts with SpoVID and MurG.

Figure 4.

(A) Silver stain SDS-PAGE of the elution fraction of the SpoVKB*-Flag immunoprecipitation from cells harvested 4 h after the induction of sporulation with anti-FLAG magnetic beads. Strains: TD883, TD884, and TD1193. (B) Immunoblot of Total (T), Flow Through (FT), Wash (W), and Elution (E) of the SpoVKB*-Flag immunoprecipitation from cells harvested 4h after the induction of sporulation with anti-Flag magnetic beads using anti-SpoVK and anti-SpoVID antisera. Strains: TD883, TD884, TD1193. (C) Subcellular localization of SpoVK-GFP in the presence or absence of SpoVID, or in cells producing SpoVID containing a defective LysM domain (T532G), or the SpoVID LysM domain alone (residues 501–575) 3.5 h after the induction of sporulation (Strains TD604, TD651, TD695, and TD1257). (D) Immunoblot of cell extracts of sporulating B. subtilis using anti-SpoVK or anti-σA antisera, from cells harvested at 4h. (Strains: PY79, KP73, KR394, JB171, JB174, TD892, and TD893). (E) Silver stain SDS-PAGE of the elution fraction of the SpoVKB*-Flag immunoprecipitation from ΔspoVID cells harvested 4 h after the induction of sporulation with anti-FLAG magnetic beads. Strains TD884 and TD1268. (F) Accumulation of peptidoglycan precursor Park’s nucleotide in strains of B. subtilis harboring WT (lanes 1–3) or defective (lanes 4–6) alleles of spoIVA, in the presence or absence (lanes 2, 5) of spoVK or the presence of spoVKA5V (lanes 3, 6).