Fig. 3 |. Species differences between human and rodent TA1.

A, Sequence alignment of residues in conserved positions within the OBP of TA1 (top), with highlighted differences between hTA1 and rTA1 (red) or mTA1 (green). Locations of residue differences between hTA1 and rTA1 (red) or mTA1 (green) are further highlighted on the structure of hTA1. Different residues in the OBP of hTA1, rTA1, and mTA1 are shown in cyan. D^3.32 is highlighted for spatial reference and Ro5256390 is shown in magenta. B, Effects of species-related mutations in the OBP on the potency and efficacy hTA1 agonists. Data are expressed as differences between mutant and wild type receptor with potencies determined as pEC50s and efficacies measured as Emax. Data represent mean ± SEM of two biological replicates. See supplementary table 1 for fitted parameter values.