Fig. 5 |. Binding pocket similarities between hTA1 and aminergic neurotransmitter receptors.

A, Side-by-side comparison of the OBP in Ro5256390-bound hTA1, the serotonin-bound 5-HT4R (PDB: 7XT8)⁴¹, the dopamine-bound DRD1 (PDB: 7F0T)⁴⁶, and the norepinephrine-bound β2AR (PDB: 4LDO)⁵³. Key residues are shown as sticks, grey dashed lines represent ionic bonds between the conserved D^3.32 residue and the aminergic ligands. B, Effects of OBP mutations on the activities of hTA1 agonists. Data are expressed as differences between mutant and wild type receptor with potencies determined as pEC50s and efficacies measured as Emax. Data represent mean ± SEM of two biological replicates. See supplementary table 1 for fitted parameter values.