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[Preprint]. 2023 Nov 7:rs.3.rs-2802998. [Version 1] doi: 10.21203/rs.3.rs-2802998/v1

Extended Data Figure 6: Nuclear-restricted FOXO1 promotes a memory-like phenotype but demonstrates impaired effector function.

Extended Data Figure 6:

a, Schematic showing a mutated variant of FOXO1 that contains three amino acid substitutions (T24A, S256A, and S319A) which restrict nuclear export (FOXO13A, orange). b, FOXO1 expression in CD19.28ζ and HA.28ζ CAR T cells from one representative donor (n = 5 donors). c, CD62L and IL7Ra expression in CD19.28ζ and HA.28ζ CAR T cells from one representative donor (n = 3 donors). d, TCF1 and LEF1 expression in CD19.28ζ CAR T cells from one representative donor (n = 3 donors). e, Cytotoxicity of HA.28ζ CAR T cells against Nalm6 leukemia (1:1 E:T, normalized to t = 0). Graph shows mean ± s.d. from one representative donor (n = 3 donors). e, 2-way ANOVA with Bonferroni’s multiple comparisons test. f, 1-way ANOVA with Dunnett’s multiple comparison’s test. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.