FIG. 2.
Organization of Telr transposon vector mini-Tn5 Tel of pJMT6 and its derivatives. This plasmid is the delivery vector for the minitransposon shown at the top. The suicide donation system used (lower part of the figure) was the pUT system (11) and included the Tn5 transposase gene devoid of NotI sites (tnp*), an Apr selection marker (bla), an origin of transfer for RP4-mediated mobilization (oriT), and the origin of replication of plasmid R6K, which is dependent on the π protein encoded by the pir gene carried by specialized λpir E. coli hosts. The top part of the figure shows the Telr cassette of pJMT4 included in the mini-Tn5 transposon vector portion of the plasmid, including a single NotI site used for cloning heterologous DNA segments. The NotI insertions used included the luxAB genes (3.2 kb) in the case of pJMT8 and the upper TOL segment (upp TOL) (12 kb, not to scale) in pJMT7.