FIG. 4.

Organization of the minimal replication-transfer segment of the pJPS plasmid series. The DNA sequence shown (length, 2,372 bp) includes a 1,815 bp SfiI-AvrII–NotI segment spanning the sequence of the repA gene and the target oriV of plasmid pMM141 (uppercase letters), as well as a 545-bp NotI–SfiI-AvrII fragment with the origin of transfer (oriT) of plasmid RK2 (lowercase letters). Depending on the specific construct (Fig. 5), these two segments may appear next to each other (as shown) or may be separated by NotI or SfiI-AvrII inserts.