FIG. 5.

Modular assembly of pJPS plasmids. The combinations of NotI and SfiI-AvrII inserts possible with the exchange of selection cassettes and cloned segments are shown. The NotI site is the preferred site for insertion of segments that originated in the previously reported cloning and expression vector pNot18, pNot19, and pVTR plasmid series (Table 1). The names of some of the combinations of these segments are indicated in the text.