TABLE 1.
Bacteria and plasmids used
| Strain or plasmid(s) | Relevant characteristics | Reference or origin |
|---|---|---|
| Strains | ||
| E. coli CC118 | Δ(ara-leu) araD ΔlacX74 galE galK phoA thi-1 rpsE rpoB argE(Am) recA1 | 11 |
| E. coli CC118λpir | CC118 lysogenized with λpir phage | 11 |
| E. coli S17-1λpir | Tpr SmrrecA thi hsdRM+, λpir phage lysogen RP4::2-Tc::Mu::Km Tn7 | 6 |
| E. coli HB101 | SmrrecA thi pro leu hsdRM+ | Lab collection |
| P. putida KT2442 | Rifr CmrhsdR+, prototrophic | 6 |
| Plasmids | ||
| RK600 | Cmr ColE1oriV RK2mob+ tra+ | 6 |
| pDT1558 | Apr, pUC8 derivative containing at its SmaI site a 3.0-kb HincII fragment from RK2 containing kilA and telAB | 32 |
| pUC18Sfi | Apr, same as pUC18 but multiple cloning site flanked by SfiI-AvrII sites | 6 |
| pUC18Not | Apr, same as pUC18 but multiple cloning site flanked by NotI sites | 6 |
| pJMT4 | Apr, pUC18Sfi containing 3.2-kb HindIII-BamHI fragment from pDT1558 containing kilA and telAB | This study |
| pSHA1 | Apr, pUC18Not containing 3.2-kb HindIII-BamHI fragment from pDT1558 containing kilA and telAB | J. Benedí |
| pUTlacZ1 | Apr Kmr, delivery vector for mini-Tn5 lacZ1 NotI flanking a kanamycin resistance gene, SfiI-AvrII sites inserted with a promoterless lacZ | 6 |
| pJMT5 | Apr Kmr, pUTlacZ1 derivative in which the lacZ SfiI-AvrII insert has been replaced by the 3.0-kb AvrII fragment of pJMT4 containing kilA and telAB | This study |
| pJMT6 | Apr Telr, pUT/mini-Tn5 Tel (NotI site free), pJMT5 derivative with an internal NotI deletion | This study |
| pJMT9 | Apr Telr, pUT/mini-Tn5 Tel (SfiI-AvrII site free), pUTlacZ derivative in which the lacZ SfiI-AvrII insert has been deleted and the kanamycin NotI inset has been replaced by the 3.0-kb NotI fragment of pSHA1 containing kilA and telAB | This study |
| pUTluxAB | Apr Tcr, delivery vector for mini-Tn5 luxAB NotI flanking a promoterless 3.2-kb luxAB reporter | 6 |
| pJMT8 | Apr Telr, pJMT6 containing the 3.2-kb NotI fragment of pUTluxAB | This study |
| pCK04AxylR | Cmr, low-copy-number plasmid with a 12-kb NotI insert containing the upp TOL catabolic segment | 6 |
| pJMT7 | Apr Telr, pJMT6 containing the 12-kb NotI fragment of pCK04AxylR | This study |
| pGP704 | Apr, oriT RK2, oriV R6K | 17 |
| pNot18 | Apr, same as pUC18 but the α-lac fragment is flanked by NotI sites | M. Herrero |
| pNot19 | Apr, same as pUC19 but the α-lac fragment is flanked by NotI sites | M. Herrero |
| pVTR-A, -B, and -C | Cmr, pSC101 replicon-based vectors with excisable expression of lacIq/Plac NotI cassette | 21 |
| pPS10 | Kmr plasmid isolated from P. syringae pv. savastanoi | 18 |
| pMM141 | Kmr, pSP10 replicon, broadened host range, replicates in E. coli and Pseudomonas strains | 7 |
| pJPS6 | Kmr, selection marker cloned as a 1.7-kb NotI insert between the RK2 oriT segment (0.5 kb) and the pMM141 minimal replicon (1.8 kb), SfiI-AvrII site available for cloning | This study |
| pJPS8 | Telr, similar to pJPS6 but selection marker replaced by the 3.0-kb NotI insert of pSHA1, SfiI-AvrII site available for cloning | This study |
| pJPS9 | Smr, selection marker cloned as a 2.1-kb SfiI insert between RK2 oriT and pMM141 oriV, NotI site available for cloning | This study |
| pJPS10 | Telr, similar to pJPS9 but selection marker replaced by the 3.0-kb AvrII insert of pJMT4, NotI site available for cloning | This study |
| pJPS11 | Telr, pJPS10 containing at its NotI site the 12-kb NotI upp TOL catabolic segment of pCK04AxylR | This study |