Figure 1.

Characteristics of antibodies from enriched antigen-specific memory B cells

(A) Every synthesized antibody was tested in duplicate by ELISA for binding against four spike antigens: RBD, S1 including RBD, S2, and the full spike protein in its trimeric form (Tri). Non-specific IgG controls were included in each ELISA and are denoted.

(B) Diversity of heavy (left) and light chain (right) of spike-reactive antibodies. Numbers above bars indicate the total antibodies represented. Only genes represented by at least 1.5% of antibodies in an individual category are shown. Where multiple antibodies per lineage were tested, only a representative single lineage is included.

(C) Proportion of sites in heavy chain V genes in spike-reactive antibodies that have experienced somatic hypermutation relative to germline. Red dots = antibodies that bind and do not neutralize pseudovirus and blue = antibodies that bind and neutralize pseudovirus. See also Figure S1 and Table S2.