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. 2023 Nov 21;223(1):e202305048. doi: 10.1083/jcb.202305048

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© 2023 Tiwari et al.

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Figure S1. — (Related to Fig. 1 and Fig. 2). Neuronal viability is sustained during glucose deprivation and hippocampal Sirt3 expression is not altered by overnight fasting. (A) Fraction of dead cells following glucose deprivation (3 h) as determined by propidium iodide staining (PI+). Mean (% total) ± SEM: +glucose (3 h), 1.76 ± 0.30; −glucose (3 h), 3.76 ± 0.53. (B) Serum glucose levels in mice fed ad lib. or fasted overnight. Serum glucose levels ± SEM (mg/dl): ad lib., 71.65 ± 10.59; fasted overnight, 16.63 ± 3.91. n = 12 mice/condition. (C) Hippocampal tissues from mice fed ad lib. or fasted overnight were immunoblotted for Sirt3 and mitochondrial ATPase5β. (D) Sirt3 band intensity normalized to ATPase5β and expressed relative to ad lib samples. Average normalized Sirt3 band intensity ± SEM: ad lib., 1.00 ± 0.13; overnight fasted, 1.354 ± 0.22. n = 11 mice/condition. Mann–Whitney U test (A, B, and D). Source data are available for this figure: SourceData FS1.