Table 2.

Key resources

Reagent or resource	Source	Identifier
Antibodies
Guinea pig anti-vGLUT1	Millipore Sigma	CAT#: AB5905
Anti-Tuj1 (β-tubulin III) antibody	R&D Systems	MAB1195
Anti-ATPase5β antibody	Sigma-Aldrich	CAT#: HPA001520
Anti-Sirt3 antibody	Cell Signaling Technology	CAT#: 5490S
Anti-β actin antibody	Bio-Rad	CAT#: HCA147P
Anti-acetylated lysine antibody	Cell Signaling Technology	CAT#: 9441S
anti-α tubulin antibody	Sigma-Aldrich	CAT#: T6074
Anti-TOMM20 antibody	Cell Signaling Technology	CAT#: 42406S
Anti-pCREB antibody	Cell Signaling Technology	CAT#: 9198S
Biological samples
rSirt3 shRNA lentivirus	Hope center viral vectors core at Washington University in St. Louis	N/A
PGC1α AAV	Gift of B. Finck	N/A
GFP AAV	Gift of B. Finck	N/A
Chemicals, peptides, and recombinant proteins
Dorsomorphin	Sigma-Aldrich	CAT#: 866405-64-3
Deacetylation inhibition cocktail	Santa Cruz	CAT#: sc-362323
6-Cyano-7nitroquinoxalibe-2, 3-dione (CNQX)	Sigma-Aldrich	CAT#: C239-25 MG
D,L-2-amino-5phosphonovaleric acid (APV)	Sigma-Aldrich	CAT#: A5282
Propidium iodide	Invitrogen	CAT#: P1304MP
Critical commercial assays
All-in-one 5X RT master mix	Applied Biosytems	CAT#: ABM-G592
PowerUp SYBR green master mix	Thermo Fisher Scientific	CAT#: A25742
TaqMan fast advanced master mix	Applied Biosytems,	CAT#: 4444557
Deposited Data
RNA-seq data	Dryad repository	Tiwari et al., 2023 Preprint
Syn-ATP analysis code	GitHub	https://github.com/ashrafilab/SynATP-Analysis
Experimental models: Cell lines
Experimental models: Organisms/Strains
Sprague-Dawley rat	Charles River	Strain code: CD IGS SD # 001 RRID: RGD_734476
C57BL/6NJ mice (overnight starvation)	Jackson Labs	Strain code: 005304 RRID:IMSR_JAX:005304
C57BL/6J mice (alternate day fasting)	Jackson Labs	Strain code: 000664 RRID:IMSR_JAX:000664
Recombinant DNA
vGLUT1-pHluorin	Voglmaier et al. (2006)	N/A
Syn-ATP	Rangaraju et al. (2014)	N/A
Synaptophysin-GCaMP6f	de Juan-Sanz et al. (2017)	N/A
mito4x-RFP	Gift of T.A. Ryan	N/A
mito4xi-GCaMP6f	Ashrafi et al. (2020)	#192075; Addgene
pLKO.1 vector	Moffat et al. (2006)	#10878; Addgene
hSirt3-RFP	This paper
Sirt3-FLAG	North et al. (2003)	#13814; Addgene
Sequence-based reagents
rSirt3 shRNA (pLKO.1 vector)	This paper	Target sequence: 5′-CTT​ACT​ACA​TGT​GGC​TGA​T-3′
qPCR primers	Source	Sequence (5′-3′)
Mouse Pgc1α forward primer	Thermo Fisher Scientific	TGA​AAA​AGC​TTG​ACT​GGC​GT
Mouse Pgc1α reverse primer	Thermo Fisher Scientific	CAA​CCA​GAG​CAG​CAC​ACT​CTA
Rat Pgc1α forward primer	Thermo Fisher Scientific	TGA​AAA​AGC​TTG​ACT​GGC​GT
Rat Pgc1α reverse primer	Thermo Fisher Scientific	CAG​GGC​AGC​ACA​CTC​TAT​GT
Rat Sirt3 forward primer	Thermo Fisher Scientific	TGT​GGG​GTC​CGG​GAG​TAT​TA
Rat Sirt3 reverse primer	Thermo Fisher Scientific	ACA​GCT​TCT​TCT​CAC​TGT​GTC​C
Rat Gapdh forward primer	Thermo Fisher Scientific	TGTGCAGTGCCAGCCTC
Rat Gapdh reverse primer	Thermo Fisher Scientific	AGA​GAA​GGC​AGC​CCT​GGT​AA
Rat 18 s Taqman probe	Thermo Fisher Scientific, #4331182	N/A
Rat Sirt3 Taqman probe	Thermo Fisher Scientific, #4331182	N/A
Rat β-actin forward primer	Thermo Fisher Scientific	GTCGAGTCCGCGTCCAC
Rat β-actin reverse primer	Thermo Fisher Scientific	TAT​CGT​CAT​CCA​TGG​CGA​ACT​GG
Software and algorithms
ImageJ	National Institute of Health	https://imagej.nih.gov/ij/
GraphPad prism 9.2.0	GraphPad Software	http://www.graphpad.com/scientific-software/prism/
Jupyter notebook	Project Jupyter	https://jupyter.org/
Adobe illustrator	Adobe, Inc.	https://www.adobe.com