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. 2023 Nov 2;26(6):540. doi: 10.3892/ol.2023.14127

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Copyright: © Chang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Effect of DHA treatment, CaMKK2-OE and NCLX siRNA on the invasive capacity of liver cancer cells. (A) Transwell assay of HepG2 cells treated with DHA, CaMKK2-OE and/or NCLX siRNA and (B) number of invasive cells per field. (C) Transwell assay of HuH-7 cells treated with DHA, CaMKK2-OE and/or NCLX siRNA and (D) number of invasive cells per field. Scale bar, 50 µm. All data are presented as the mean ± standard deviation (n=3). Data were analyzed using one-way analysis of variance followed by Bonferroni post hoc test. **P<0.01; ***P<0.001. NCLX, mitochondrial sodium/calcium exchanger protein; DHA, dihydroartemisinin; CaMKK2, calcium/calmodulin-dependent protein kinase kinase 2; siRNA, small interfering RNA; OE, overexpression; ns, not significant; NC, negative control.