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. 2023 Nov 2;26(6):540. doi: 10.3892/ol.2023.14127

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Copyright: © Chang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 7. — Effects of DHA treatment and CaMKK2-OE on ATP synthase expression. Relative mRNA expression levels of (A) ATP1A1, (B) ATP5H, (C) CaMKK2 and (D) NCLX in HepG2 cells transfected with CaMKK2-OE and treated with DHA were detected by RT-qPCR. Relative mRNA expression levels of (E) ATP1A1, (F) ATP5H, (G) CaMKK2 and (H) NCLX in HuH-7 cells transfected with CaMKK2-OE and treated with DHA were detected by RT-qPCR. All data are presented as the mean ± standard deviation (n=3). Data were analyzed using one-way analysis of variance followed by Bonferroni post hoc test. *P<0.05; **P<0.01; ***P<0.001. NCLX, mitochondrial sodium/calcium exchanger protein; DHA, dihydroartemisinin; CaMKK2, calcium/calmodulin-dependent protein kinase kinase 2; OE, overexpression; RT-qPCR, reverse transcription-quantitative PCR; NC, negative control; ATP1A1, sodium/potassium-transporting ATPase subunit α-1; ATP5H, ATP synthase subunit d, mitochondrial.