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. 2023 Nov 2;26(6):540. doi: 10.3892/ol.2023.14127

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Copyright: © Chang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 8. — Effects of DHA treatment and CaMKK2-OE on ATP synthase expression. (A) Western blotting and protein expression levels of (B) ATP1A1, (C) ATP5H, (D) CaMKK2 and (E) NCLX in HepG2 cells transfected with CaMKK2-OE and treated with DHA. (F) Western blotting and protein expression levels of (G) ATP1A1, (H) ATP5H, (I) CaMKK2 and (J) NCLX in HuH-7 cells transfected with CaMKK2-OE and treated with DHA. All data are presented as the mean ± standard deviation (n=3). Data were analyzed using one-way analysis of variance followed by Bonferroni post hoc test. **P<0.01; ***P<0.001. NCLX, mitochondrial sodium/calcium exchanger protein; DHA, dihydroartemisinin; CaMKK2, calcium/calmodulin-dependent protein kinase kinase 2; OE, overexpression; NC, negative control; ATP1A1, sodium/potassium-transporting ATPase subunit α-1; ATP5H, ATP synthase subunit d, mitochondrial.