FIG. 1.

Specificity of the S. iniae PCR assay. Agarose gel (3%)-resolved ethidium bromide-stained PCR products from template DNA of the following needle-touched bacterial colonies. Lane 1, S. iniae ATCC 29178^T; lane 2, S. iniae ND 2-16; lane 3, isolate KFP 109 (sea bream); lane 4, isolate KFP 101 (European sea bass); lane 5, isolate KFP 110 (wild spine foot); lane 6, S. agalactiae ATCC 13813; lane 7, Lactococcus garvieae ATCC 43921^T; lane 8, Vagococcus salmoninarum NCFB 2777^T; lane 9, L. lactis NCFB 604^T; lane 10, S. parauberis ATCC 13387; lane 11, L. piscium NCFB 2778^T; lane 12, Aeromonas salmonicida 3173/86; N, negative control (no DNA); M, DNA molecular weight marker VI. The arrow indicates the expected length of 300 bp.