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Validation phase. Six miRNAs (A–F) differentially expressed in small RNA sequencing and replicated in the discovery cohort were assessed with RT-qPCR in whole blood samples of 59 hypertension subjects and 55 controls of the validation cohort. Expression levels of miRNAs are expressed as the number of copies per ng of RNA and are shown as scatterplots indicating mean ± standard deviation. p-values from the Mann–Whitney Rank Sum test are indicated.
