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. 2023 Oct 31;5(4):639–650. doi: 10.3390/clockssleep5040043

Figure 2.

Figure 2

Guanabenz shortens the circadian period and increases the amplitude of Per2-dLuc rhythms in 3T3 fibroblasts: (A) Representative traces of bioluminescence recordings. For this experiment, 3T3 Per2-dLuc fibroblasts were treated with 5 µM or 10 µM guanabenz, or the vehicle (DMSO), and bioluminescence recordings were performed using the LumiCycle instrument. (B) Circadian period of Per2-dLuc rhythms. Note that guanabenz treatment shortened the circadian period (F(2,6) = 9.451, p = 0.0140, one-way ANOVA; Vehicle vs. 5 μM, t = 3.597, p = 0.0342; Vehicle vs. 10 μM, t = 3.913, p = 0.0236; Bonferroni’s post hoc comparisons). (C) Amplitude of Per2-dLuc rhythms. Note that guanabenz treatment increased the amplitude (F(2,6) = 11.25, p = 0.0093, one-way ANOVA; Vehicle vs. 5 μM, t = 3.942, p = 0.0228; Vehicle vs. 10 μM, t = 4.255, p = 0.0161; Bonferroni’s post hoc comparisons). Data are shown as individual values and mean ± SEM. * p < 0.05.