Table 1.
Semi-quantitative evaluation of the response to different CFTR modulators and their combination
| SOA increase (rectal) | FIS (nasal + rectal) | Isc (nasal + rectal) | WB (nasal + rectal) | |
|---|---|---|---|---|
| VX770 | NA | ++ | +/− | NA |
| VX809 | – | +/− | +/− | + |
| VX809 + VX770 | NA | ++ | +/− | NA |
| VX661 | + | + | + | + |
| VX661 + VX770 | NA | + | + | NA |
| VX445 + VX661 | + | + | ++ | ++ |
| VX445 + VX661 + VX770 | NA | + | ++ | NA |
No (−), very low (+/−), measurable (+), good (++) responses; Note that VX770 was added at the time of assay; SOA: steady-state organoid area; NA: not assayed; FIS: forskolin-induced swelling assay, Isc: short circuit currents (Ussing chamber), WB: western blot.