Figure 2.

Long 5′-tRFs and 5′-tRHs from the nuclear trna116_GluCTC (hg19: chr1, reverse strand, 145399233-145399304) in colon tissues and model cell lines. In patient colon samples and colorectal cell lines, fragments vary in abundance based on their length and the nature of the biosample. Samples SRR15214273 and SRR15214274 correspond to knockdown of the RAN binding protein 1 (RANBP1). Samples SRR7903704, SRR7903705, and SRR7903706 are RIP-seq experiments using an antibody that recognizes RNAs with methylation of guanosine at position 7 (m7G). The other samples were untreated. tRFs with lengths 32–35 nts are 5′-tRHs (bold, yellow). In the sequence, the anticodon is emphasized (bold, underline). Datasets are identified by their NCBI Sequence Read Archive (SRA) bioproject (second row) and Sequence Read Run (SRR) numbers (third row). Fragments with abundance < 10 reads per million (RPM) were thresholded and are displayed as having an abundance of 0 RPM. Data coloring follows a linear scale up to 1000 RPM.