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. 2023 Oct 18;12(11):3366–3380. doi: 10.1021/acssynbio.3c00367

Figure 5.

Figure 5

Production of an unnatural polyketide by engineered heterologous hosts. (a) Serine recombinase-assisted integration of the methylmalonyl-CoA mutase (mut) and epimerase (epi) pathway into P. putida AG5577. mmCoA: methylmalonyl-CoA. (b) Engineered pathway for the production of the LipPKS loading substrate isobutyryl-CoA by the heterologous enzymes Kivd (ketoisovalerate decarboxylase) and CCL4 (2-methylpropanoate–CoA ligase) in C. glutamicum AG6212. Isobutyric and propionic acids were added to the C. glutamicum production medium (green). aldh: endogenous aldehyde dehydrogenase activity. (c) Regression plot of the LipPKS counts and polyketide production levels in the engineered hosts C. glutamicum AG6212cz, E. coli K207–3, and P. putida AG5577mm (n = 3). The calculation of the Pearson correlation included every replicate as a single data point. Target host optimization: Cg = C. glutamicum; Ec = E. coli; Pp = P. putida; Optimization strategy: ubc = use best codon; mcu = match codon usage; hrca = harmonize relative codon adaptiveness.