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. 2023 Oct 18;12(11):3366–3380. doi: 10.1021/acssynbio.3c00367

Table 1. Plasmids and Strains Used in This Study.

plasmid description reference JBEI part ID
pJH204 BxB1 integration vector containing BxB1 attP site. Kanamycin selection marker and ColE1 origin flanked by ΦC31 attB and attP site. (24)  
pBH026 Derivative of BxB1 integration vector pJH204 with promoter LlacO1 repressed by LacI. Kanamycin selection marker, ColE1 origin, and lacI are flanked by ΦC31 attB and attP site. This work  
pBH026 RFP BxB1 integration vector with rfp gene This work JPUB_021284
pK18 Suicide vector for allelic replacement with KanR, SacB (55)  
pK18 ΔprpDBC2 Suicide vector for in-frame deletion of prpDBC2 (34)  
pK18 ΔCgl0605::kivd-CCL4 Suicide vector for replacement of Cgl0605 with kivd from Lactococcus lactis and CCL4 from Humulus lupulus This work JPUB_021290
pK18 ΔCgl1016::sfp Suicide vector for replacement of Cgl1016 with sfp from Bacillus subtilis (34)  
pBH026 LipPKS-Cg_ubc BxB1 integration vector with ubc codon optimized LipM1+TE gene for expression in C. glutamicum This work JPUB_021239
pBH026 LipPKS-Cg_mcu BxB1 integration vector with mcu codon optimized LipM1+TE gene for expression in C. glutamicum This work JPUB_021241
pBH026 LipPKS-Cg_hrca BxB1 integration vector with hrca codon optimized LipM1+TE gene for expression in C. glutamicum This work JPUB_021243
pBH026 LipPKS-Pp_ubc BxB1 integration vector with ubc codon optimized LipM1+TE gene for expression in P. putida This work JPUB_021306
pBH026 LipPKS-Pp_mcu BxB1 integration vector with mcu codon optimized LipM1+TE gene for expression in P. putida This work JPUB_021246
pBH026 LipPKS-Pp_hrca BxB1 integration vector with hrca codon optimized LipM1+TE gene for expression in P. putida This work JPUB_021248
pBH026 LipPKS-Ec_ubc BxB1 integration vector with ubc codon optimized LipM1+TE gene for expression in E. coli This work JPUB_021250
pBH026 LipPKS-Ec_mcu BxB1 integration vector with mcu codon optimized LipM1+TE gene for expression in E. coli This work JPUB_021237
pBH026 LipPKS-Ec_hrca BxB1 integration vector with hrca codon optimized LipM1+TE gene for expression in E. coli This work JPUB_021263
pAN001 Derivative of pBH026 with additional URA3 marker for yeast assembly This work  
pAN001 LipPKS-WT_ATG BxB1 integration vector with wildtype LipM1+EryM6-TE nucleotide sequence and ATG start codon This work JPUB_021267
pAN001 LipPKS-WT_GTG BxB1 integration vector with wildtype LipM1+EryM6-TE nucleotide sequence and GTG start codon This work JPUB_021265
pJH209 MR11 integration vector containing MR11 attP site. Kanamycin selection marker and ColE1 origin are flanked by ΦC31 attB and attP site. (24)  
pJH209 Sc_mmCoA MR11 integration vector with mmCoA pathway from S. cellulosum So56 under the control of Ptac This work JPUB_021252
pGW30 ColE1 vector with apramycin marker expressing ΦC31 integrase under the control of Ptac (24)  
pALC412 Derivative of pGW30 with optimized expression of ΦC31 integrase for use in C. glutamicum Adam Guss, personal communication  
pGW31 ColE1 vector with apramycin marker expressing BxB1 integrase under the control of Ptac (24)  
pGW36 ColE1 vector with apramycin marker expressing MR11 integrase under the control of Ptac (24)  
pGingerBG-NahR BBR1 vector with gentamicin marker containing the Psal/NahR inducible system (56)  
pGingerBG-NahR LipPKS-Pp_mcu Derivative of pGingerBG-NahR carrying LipPKS-Pp_mcu This work JPUB_021282
pGingerBG-NahR LipPKS-Ec_mcu Derivative of pGingerBG-NahR carrying LipPKS-Ec_mcu This work JPUB_021280
strain description reference JBEI part ID
E. coli XL1 Blue   Agilent  
E. coli BL21(DE3)   NEB  
  Carrying replicating vector pBH026 LipPKS-Cg_ubc This work  
  Carrying replicating vector pBH026 LipPKS-Cg_mcu This work  
  Carrying replicating vector pBH026 LipPKS-Cg_hrca This work  
  Carrying replicating vector pBH026 LipPKS-Pp_ubc This work  
  Carrying replicating vector pBH026 LipPKS-Pp_mcu This work  
  Carrying replicating vector pBH026 LipPKS-Pp_hrca This work  
  Carrying replicating vector pBH026 LipPKS-Ec_ubc This work  
  Carrying replicating vector pBH026 LipPKS-Ec_mcu This work  
  Carrying replicating vector pBH026 LipPKS-Ec_hrca This work  
  Carrying replicating vector pAN001 LipPKS-WT_ATG This work  
  Carrying replicating vector pAN001 LipPKS-WT_GTG This work  
  Carrying replicating vector pBH026 RFP This work  
E. coli K207–3 Derivative of sfp-expressing E. coli strain BAP1 containing the PCCase pathway from S. coelicolor for the production of mmCoA. Both heterologous pathways are under the control of the T7 promoter. (29)  
  Carrying replicating vector pBH026 LipPKS-Cg_ubc This work  
  Carrying replicating vector pBH026 LipPKS-Cg_mcu This work  
  Carrying replicating vector pBH026 LipPKS-Cg_hrca This work  
  Carrying replicating vector pBH026 LipPKS-Pp_ubc This work  
  Carrying replicating vector pBH026 LipPKS-Pp_mcu This work  
  Carrying replicating vector pBH026 LipPKS-Pp_hrca This work  
  Carrying replicating vector pBH026 LipPKS-Ec_ubc This work  
  Carrying replicating vector pBH026 LipPKS-Ec_mcu This work  
  Carrying replicating vector pBH026 LipPKS-Ec_hrca This work  
  Carrying replicating vector pAN001 LipPKS-WT_ATG This work  
  Carrying replicating vector pAN001 LipPKS-WT_GTG This work  
AG6212 Derivative of C. glutamicum ATCC13032 with poly attB site replacing Cgl1777–8 Adam Guss, personal communication  
  BxB1 integrated pBH026 RFP This work JPUB_021285
  BxB1 integrated pBH026 RFP with excised vector backbone This work JPUB_021287
AG6212cz Derivative of AG6212 with the following modifications: ΔprpDBC2ΔCgl0605::kivd-CCL4ΔCgl1016::sfp This work JPUB_021253
  BxB1 integrated pBH026 LipPKS-Cg_ubc This work JPUB_021254
  BxB1 integrated pBH026 LipPKS-Cg_mcu with excised vector backbone This work JPUB_021255
  BxB1 integrated pBH026 LipPKS-Cg_hrca with excised vector backbone This work JPUB_021256
  BxB1 integrated pBH026 LipPKS-Pp_ubc with excised vector backbone This work JPUB_021257
  BxB1 integrated pBH026 LipPKS-Pp_mcu with excised vector backbone This work JPUB_021258
  BxB1 integrated pBH026 LipPKS-Pp_hrca with excised vector backbone This work JPUB_021259
  BxB1 integrated pBH026 LipPKS-Ec_ubc This work JPUB_021260
  BxB1 integrated pBH026 LipPKS-Ec_mcu with excised vector backbone This work JPUB_021261
  BxB1 integrated pBH026 LipPKS-Ec_hrca with excised vector backbone This work JPUB_021262
  BxB1 integrated pAN001 LipPKS-WT_ATG with excised vector backbone This work JPUB_021267
  BxB1 integrated pAN001 LipPKS-WT_GTG with excised vector backbone This work JPUB_021264
AG5577 Derivative of P. putida KT2440 with three triple attB sites replacing PP_4740 and PP_2876 and integrated between PP_4217/PP_4218, respectively Adam Guss, personal communication  
  BxB1 integrated pBH026 RFP This work JPUB_021283
  BxB1 integrated pBH026 RFP with excised vector backbone This work JPUB_021286
  BxB1 integrated pBH026 LipPKS-Cg_ubc with excised vector backbone This work JPUB_021238
  BxB1 integrated pBH026 LipPKS-Cg_mcu with excised vector backbone This work JPUB_021240
  BxB1 integrated pBH026 LipPKS-Cg_hrca with excised vector backbone This work JPUB_021242
  BxB1 integrated pBH026 LipPKS-Pp_ubc with excised vector backbone This work JPUB_021244
  BxB1 integrated pBH026 LipPKS-Pp_mcu with excised vector backbone This work JPUB_021245
  BxB1 integrated pBH026 LipPKS-Pp_hrca with excised vector backbone This work JPUB_021247
  BxB1 integrated pBH026 LipPKS-Ec_ubc with excised vector backbone This work JPUB_021249
  BxB1 integrated pBH026 LipPKS-Ec_mcu This work JPUB_021236
  BxB1 integrated pBH026 LipPKS-Ec_hrca This work JPUB_021292
  BxB1 integrated pAN001 LipPKS-WT_ATG with excised vector backbone This work JPUB_021294
  BxB1 integrated pAN001 LipPKS-WT_GTG with excised vector backbone This work JPUB_021293
AG5577mm MR11 integrated pJH209 Sc_mmCoA for the production of mmCoA. This work JPUB_021251
  BxB1 integrated pBH026 LipPKS-Cg_ubc with excised vector backbone This work JPUB_021295
  BxB1 integrated pBH026 LipPKS-Cg_mcu with excised vector backbone This work JPUB_021296
  BxB1 integrated pBH026 LipPKS-Cg_hrca with excised vector backbone This work JPUB_021297
  BxB1 integrated pBH026 LipPKS-Pp_ubc with excised vector backbone This work JPUB_021298
  BxB1 integrated pBH026 LipPKS-Pp_mcu with excised vector backbone This work JPUB_021299
  BxB1 integrated pBH026 LipPKS-Pp_hrca with excised vector backbone This work JPUB_021300
  BxB1 integrated pBH026 LipPKS-Ec_ubc with excised vector backbone This work JPUB_021301
  BxB1 integrated pBH026 LipPKS-Ec_mcu This work JPUB_021302
  BxB1 integrated pBH026 LipPKS-Ec_hrca This work JPUB_021303
  BxB1 integrated pAN001 LipPKS-WT_ATG with excised vector backbone This work JPUB_021305
  BxB1 integrated pAN001 LipPKS-WT_GTG with excised vector backbone This work JPUB_021304