Figure 3.

G2019S pMac antigen presentation and lysosomal phenotypes are rescued by knockdown of LRRK2

pMacs from 10- to 12-week-old male B6, WTOE, or G2019S mice were nucleofected with a Lrrk2-targeting ASO or control ASO and stimulated with 100 U IFNγ and harvested at 2, 6, or 18 h. (A and B) Lrrk2 protein levels normalized to β-actin levels and quantified. (C) YAe MFI was quantified on LPMs using flow cytometry. (D) Surface MHC-II GMFI was quantified in LPMs using flow cytometry. (E) DQ-BSA MFI was quantified in LPMs using flow cytometry. (F) BMV109 MFI was quantified in LPMs using flow cytometry. Bars represent mean ± SEM (n = 8–10). Two- or three-way ANOVA, Bonferroni post hoc test, groups sharing the same letters are not significantly different (p > 0.05), and groups displaying different letters are significantly different (p < 0.05).