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. 2023 Oct 20;34:102064. doi: 10.1016/j.omtn.2023.102064

Figure 4.

Figure 4

Lrrk2 kinase inhibition and knockdown via ASO reduces trafficking of MHC-II to the plasma membrane

pMacs from 10- to 12-week-old male B6, WTOE, or G2019S mice were stimulated with 100 U IFNγ for 18 h and stained for intracellular and extracellular MHC-II MFI, and exMHCII:icMHCII ratio was quantified. White arrows indicate perinuclear clustering of icMHCII. Scale bars, 30 μM (A and B). Percentage of cells with perinuclear clustering was quantified (C). pMacs from 10- to 12-week-old male B6, WTOE, or G2019S mice were nucleofected with a Lrrk2-targeting ASO or control ASO and stimulated with 100 U IFNγ for 18 h and stained for intracellular and extracellular MHC-II MFI, and exMHCII:icMHCII ratio was quantified. White arrows indicate perinuclear clustering of icMHCII. Scale bars, 30 μM (D and E). Bars represent mean ± SEM (n = 6). two-way ANOVA, Bonferroni post hoc test, groups sharing the same letters are not significantly different (p > 0.05), and groups displaying different letters are significantly different (p < 0.05).