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. 2023 Oct 20;34:102064. doi: 10.1016/j.omtn.2023.102064

Figure 5.

Figure 5

LRRK2 knockdown via antisense oligonucleotide and kinase inhibition alters cytokine release from pMacs

pMacs from 10- to 12-week-old male B6, WTOE, or G2019S mice were nucleofected with a Lrrk2-targeting ASO or control ASO and stimulated with 100 U IFNγ, or were plated with 100 U IFNγ ± 100 nM of Pf360 and media collected after 18 h. Cytokine levels of IL-10 (A and B), TNF (C and D), IL12p70 (E), and IL-4 (F) were quantified and normalized to live cell count. Bars represent mean ± SEM (n = 8–10). Two-way ANOVA, Bonferroni post hoc test, groups sharing the same letters are not significantly different (p > 0.05), and groups displaying different letters are significantly different (p < 0.05).