Figure 6.

ASO-mediated Lrrk2 knockdown alters critical autophagy and cytokine signaling pathways in G2019S and WTOE pMacs

Transcriptomic analysis from WTOE or G2019S vehicle and IFNγ-treated pMacs nucleofected with control or Lrrk2 ASO. (A) Normalized log₂(count) value for Lrrk2 mRNA was plotted. Bars represent mean ± SEM (n = 6). Two-way ANOVA, Bonferroni post hoc test, groups sharing the same letters are not significantly different (p > 0.05), and groups displaying the same letter are significantly different (p < 0.05). (B and C) Significant control ASO:Lrrk2 ASO DEGs in G2019S pMacs were counted and compared across vehicle and IFNγ treatments. (D and E) Significant control ASO:Lrrk2 ASO DEGs in WTOE pMacs were counted and compared across vehicle and IFNγ treatments. Volcano plots show proteins with fold change > 1.5 and adjusted p ≤ 0.05. (F) ShinyGO version 0.76.3 was used to identify pathways in which significant DEGs were associated with. (G) Heatmaps show DEGs seen only in G2019S pMacs treated with IFNγ. (H) ShinyGO version 0.76.3 was used to identify pathways in which significant DEGs were associated with. (I) Heatmaps show DEGs seen only in WTOE pMacs treated with IFNγ.