The anti-ASFV effect and IC50 of TET. (A) The anti-ASFV effect of TET on PAMs was analyzed by IFA. Bar = 100 μm. (B-D) The anti-ASFV effect of TET on PAMs, BMDMs and MA104 cells was analyzed by Western Blot. (E) IC50 of TET in PAMs, BMDMs and MA104 cells was calculated. Inhibition rate was calculated by Cell-ELISA assay. (F-I) TET reduced infection of different MOI ASFV. TET-preincubated PAMs were incubated with ASFV at an MOI of 0.5, 1, 2, or 5. The expression levels of ASFV-p72 were analyzed by Western Blot.