Fig. 5.

ASFV induces Akt phosphorylation during internalization. The level of Akt phosphorylation was detected by Western Blot at the indicated time points. (A) The results in PAMs show a significant increase in the level of Akt phosphorylation at three time points post-infection (5 min, 10 min and 20 min), compared to other time points. (B) The results in MA104 cells indicate a significant increase in the level of Akt phosphorylation at four time points post-infection (10 min, 20 min, 30 min and 1 h), compared to other time points. (C) Virus attachment tests were performed on MA104 cells. Western Blot showed that ASFV did not induce Akt phosphorylation during attachment. (D) The phosphorylation level of Akt in the TET treated group was significantly reduced after 20 min, indicating that TET inhibits phosphorylation of Akt and blocks the activation of PI3K/Akt pathway.