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. 2023 Nov 20;14:7538. doi: 10.1038/s41467-023-43178-2

Fig. 6. CRISPR–Cas9-mediated inactivation of three homoeoalleles of LHP1 confers resistance to stripe rust.

Fig. 6

a Pst growth in wild-type and Talhp1-abd plants at 2 dpi (stained with wheat germ agglutinin conjugated to Alexa-488); bar, 1 cm. b Left: wild-type and Talhp1-abd plants inoculated with Pst CYR32 and photographed at 14 dpi; bar, 50 µm. Right: quantification of Pst growth in infected JW1 and Talhp1-abd leaves at 2 dpi by wheat germ agglutinin staining. Values represent the mean ± SD (error bars) (n = 40). For JW1, mean value = 1563, median = 1468, SEM = 100, SD = 629; For Talhp1-abd, mean value = 1227, median = 1101, SEM = 75, SD = 472. Horizontal lines in boxplots show median, hinges show IQR, whiskers show 1.5 × IQR, points beyond 1.5 × IQR past hinge are shown. c Left: H2O2 accumulation in wheat leaves at 2 dpi revealed by DAB staining; bar, 20 µm. Right: quantification of H2O2 accumulation in infected JW1 and Talhp1-abd leaves at 2 dpi by DAB staining (n = 40). For JW1, mean value = 1999, median = 1683, SEM = 178, SD = 1128; For Talhp1-abd, mean value = 2945, median = 2696, SEM = 234, SD = 1481. Boxplots definition is the same as (b).