Figure 1.

Bioinformatics analysis combined with PCR was used to verify the expression of EGR2 and LAG3 in patients with SSc-ILD

(A)The expressions of EGR2 and LAG3 in lung tissue of SSc-ILD patients were decreased. Data are represented as the form of boxplots. Statistical significance was analyzed by unpaired two-tail t-test, ∗∗p < 0.01.

(B–D) There was no significant difference between LAG3 and M0 and M1 macrophages, but LAG3 was significantly correlated with M2 macrophages. Data are represented as the form of correlation scatterplots. Statistical significance was analysis by chi-square test for comparison between groups.

(E and F) The expression of EGR2 and LAG3 was decreased in peripheral blood of patients with SSc-ILD. Data are represented as mean ± SD. Statistical significance was analyzed by one-way ANOVA (n = 3), ∗p < 0.05.