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. 2023 Oct 17;26(12):108225. doi: 10.1016/j.isci.2023.108225

Figure 3.

Figure 3

CD4+LAG3+T cells can inhibit TGF-β1-induced activation and mesenchymal transition of lung fibroblasts

(A) Flow cytometry shows that the percentage of CD4+LAG3+T cells isolated by magnetic beads increased from 2.83% to 32.89%.

(B) ELISA results show that the control group and CD4+LAG3−T cells did not significantly secrete TGF-β3, while CD4+LAG3+T cells could secrete high levels of TGF-β3. Data are represented as mean ± SD. Statistical significance was analyzed by one-way ANOVA (n = 3), ∗∗∗∗p < 0.0001.

(C–G) The supernatant of CD4+LAG3+T cells can inhibit collagen secretion, proliferation, migration, invasion, mesenchymal transition, and F-actin expression of pHLFs stimulated by TGF-β1, while antagonistic TGF-β3 can weaken these effects. Data are represented as mean ± SD. Statistical significance was analyzed by one-way ANOVA (n = 5), ∗p < 0.05, ∗∗p < 0.01.