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. 2023 Nov 20;9:87. doi: 10.1038/s41522-023-00454-9

Fig. 2. HMbmouse vaginal microbiota contains distinct taxa compared to conventional mice and is dynamic within the colony and over time.

Fig. 2

Vaginal swabs were collected from separate cohorts of HMbmice over the course of two years. a Vaginal microbial compositions of distinct cohorts with the duration since previous sampling noted below. Samples from Cohort 1 – Cohort 3 (n = 10−27) represent baseline vaginal swabs from unique mice. Cohort 4 and 5 (n = 12−23) were swabbed repeatedly at baseline over the course of a week; all samples are displayed. b PCoA of vaginal communities clustered by weighted normalized UniFrac distances between mice in different cohorts. Centroids were determined by jackknifed rarefaction (100 reads). Dissimilarity between consecutive cohorts using (c) Bray-Curtis and (d) weighted normalized UniFrac distances. e Top 20 important features over the course of repeated sampling of Cohort 4 and 5 and (f) the net average change in feature relative abundance. g Changes in vaginal community OTU with increased age. Each column (a) or symbol (b, g) represents a unique vaginal swab community. Tukey’s boxplots are displayed (c−d). Values for each feature are displayed (e−f) and colored as in (a). Data were statistically analyzed by PERMANOVA followed by PERMDISP (c−d), Random Forest Regression on 50 estimators (e), and Spearman Correlation (g). PERMANOVA P values (P < 0.1) are colored in red if PERMDISP was also statistically significant (P < 0.05). All statistically significant P values are reported.