Fig. 4. ^HMbmice exhibit distinct community state types that vary in frequency by estrous stage.

Vaginal swabs were collected and categorized by both estrous stage and composition. a Community state type categorization for ^HMbmice (n = 183 samples) hierarchically clustered into humanized murine CST (upper bar) and its associated estrous stage (lower bar). Estrous staged (n = 35) ^HMbmice from Figs. 2 and 3 and unstaged (n = 64) ^HMbmice from Fig. 2 are depicted. b Prevalence of ^hmCSTs in each estrous stage with total samples per stage noted above. c ^hmCST transition rates from starting community (left) to community three days later (top) with total numbers per starting ^hmCST noted on the right. PCoA of vaginal communities clustered by weighted normalized UniFrac distances between mice assigned different (d) estrous stage and (e) ^hmCST. Centroids were determined by jackknifed rarefaction (100 reads). f Weighted normalized UniFrac distances of microbial compositions between ^hmCSTs. ^hmCSTs labeled below are compared to the ^hmCST of the corresponding background color. Each column (a) or symbol (d−e) represents a unique vaginal swab community. Individual comparisons marked as symbols (f) are displayed with median and interquartile ranges. Data were analyzed by Chi Square analysis of fractions (b) or PERMANOVA followed by PERMDISP (f). PERMANOVA P values (P < 0.1) are colored in red if PERMDISP was also statistically significant (P < 0.05). Statistically significant comparisons are noted in the main text (b); ⁺P < 0.03; ⁺⁺P < 0.003.