Figure 3.

Packaging fitness of AAV domain insertion libraries mapped to the capsid structure

(A) Top left corner: AAV-DJ capsid structure view from the inside (left) and outside (right) radially color cued. The 2-, 3-, and 5-fold axes are indicated. VP1u domain was modeled using RoseTTAFold⁹⁸ and manually positioned. All other structures show packaging fitness heatmaps of the indicated domain insertions. Green indicates higher and magenta lower fitness than AAV-DJ (RCSB PDB: 7KFR). (B) Zoom of the outside structures from (A). The 2-, 3-, and 5-fold axes are outlined.