Figure 5.

Variance of packaging and uptake fitness

(A and B) Variance of packaging fitness (A) and uptake fitness (B) from all domain insertion libraries mapped to the AAV capsid structure. The capsid inside (top) and the capsid outside (bottom) are shown (RCSB PDB: 7KFR). (C and D) Empirical cumulative density insertional fitness of residues within (petrol green) and outside (red) the 2-fold axis (C) and 3-fold axis (D). Significance of distribution differences was tested using a two-sided, two-sample Kolmogorov-Smirnov test. Significance level and p values are shown. (E) Venn diagram showing which of the 335 AAV interface residues are unique and shared among interfaces.