Figure 6.

Unbiased clustering of insertion fitness

(A) UMAP cluster analysis of the AAV domain insertional profiling data resulting in five distinct clusters. (B) Cluster map to distinct capsid regions: (1) N terminus of VP1 and bases of the 3-fold and 5-fold interface in red; (2) the HI loop, the pore and the inner connecting residue layer in yellow; (3) protrusions of the 3-fold interface in green; (4) 2-fold axis in blue; and (5) the surrounding of the 5-fold pore in light lilac. (C) Mean domain insertion fitness of packaging, pull-down, binding, and uptake shown by residues aligned to the UMAP clusters in (A). (D) Distribution of insertion fitness of packaging, pull-down, and uptake for each cluster.