Alignment of the PCR product sequences overlapping the V4 domains of 10 species of the genus Agrocybe. The beginning and end of the variable domain are indicated by arrows. Asterisks indicate nucleotides that are strictly conserved in the 10 species. The locations and putative sizes of insertion-deletion events are indicated by dashes. Nucleotides that are strictly conserved in the 10 species are enclosed in shaded boxes. The nine nucleotides constituting an inverted repeated sequence at the boundaries of the insertion-deletion site in A. chaxingu are indicated by boldface type. The boxes labeled V4a, V4b, and V4c are the locations of putative insertion-deletion events. The GenBank accession numbers for the sequences are as follows: A. aegerita, AF080400; A. alnetorum, AF080401; A. chaxingu, AF080402; A. dura, AF080403; A. erebia, AF080404; A. firma, AF080405; A. paludosa, AF080406; A. pediades, AF080407; A. praecox, AF080408; and A. vervacti, AF080409.