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. Author manuscript; available in PMC: 2023 Nov 21.
Published in final edited form as: Brain Behav Immun. 2022 Feb 14;102:110–123. doi: 10.1016/j.bbi.2022.02.008

Fig. 6. The impact of PEA on immune activation, mast cell degranulation, and BBB integrity in the hippocampus of HFD mice.

Fig. 6.

Western blot for (A) TLR4, and Real-Time PCR analysis of (B) Myd88, and (C) Tlr2, (D) Cma1, (E) Tpsb2 (F) Tjp1, (G) Ocln, and (H) Cldn5 in the hippocampus of all experimental groups (at least n = 5 per group for western blot; n = 5 per group for Real-Time PCR). Morphometric analysis of albumin-immunoreactive cells in hippocampal (I) DG and (J) CA1 subfield. As shown in the upper panels of I and J, most albumin-positive cells (green, arrowheads) are also positive for the neuronal marker NeuN (red, arrowheads). The lower panels of I and J represent images depicting albumin staining in the DG and, respectively, CA1 subfield for each experimental condition. Hil, hilus; Py, pyramidal cell layer. In the merged images, cell nuclei are stained with TO-PRO3. The semiquantitative evaluation of albumin-positive neuronal-like profiles (n = 5 per group) is also shown. All data are shown as mean ± S.E.M. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.